Notice In vitro research only · Not for human consumption
Free shipping from 200 €
EuroPeptiva

Reconstitution calculator

Tell us how many milligrams are in the vial, how much bacteriostatic water you add and how much you want to draw: we give you the resulting concentration and how far that aliquot reaches on the syringe scale.

Milligrams in the vial
Bacteriostatic water you add

Every lyophilized vial ships with a free 3 ml vial of bacteriostatic water, so that is almost always the starting volume.

Aliquot you want to draw
Total syringe volume

A syringe graduated in units (U-100) is marked 100 units per millilitre: 0.5 ml is 50 units.

To draw 0.50 mg

fill the syringe to

15.0 units

Vial concentration
3.33 mg/ml
Aliquot volume
0.150 ml
Aliquots per vial
20

The result is arithmetic, not a usage recommendation. EuroPeptiva products are intended exclusively for in vitro scientific research and are not suitable for human or veterinary consumption.

How it is worked out

  1. The concentration is the milligrams in the vial divided by the millilitres of water you add. A 10 mg vial with 3 ml of water ends up at 3.33 mg/ml.
  2. The aliquot volume is the milligrams you want to draw divided by that concentration. 0.5 mg at 3.33 mg/ml is 0.15 ml.
  3. The syringe units are that volume times one hundred, because a U-100 syringe is marked 100 units per millilitre. 0.15 ml is 15 units.

The water you add does not change how much peptide is in the vial, only how much liquid it is spread through: with twice the water, the same aliquot takes up twice the volume in the syringe. That is why it pays to pick a volume that leaves the mark somewhere comfortable to read.

Short of something to reconstitute with?

Bacteriostatic water, syringes and alcohol wipes, all in the same order.

View the catalogue