Gonadotropins
Kisspeptin-10
C-terminal decapeptide of kisspeptin, an agonist of the KISS1R (GPR54) receptor, researched in reproductive-axis signalling.
| Purity | >98% |
| Molecular formula | C63H83N17O14 |
| Molecular weight | 1302.40 Da |
| CAS number | 374675-21-5 |
Research background
Kisspeptin is a peptide encoded by the KISS1 gene, and its signal is received by the KISS1R receptor, formerly known as GPR54. Kisspeptin-10 is the C-terminal decapeptide (metastin 45-54, sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2): the minimal fragment that keeps the ability to activate the receptor.
Its described role is upstream of the gonadotropic axis: the kisspeptin/KISS1R system regulates the neurons that release gonadotropin-releasing hormone (GnRH), which in turn govern the secretion of LH and FSH. The identification of metastin as the ligand of GPR54 was published in 2001 (Ohtaki et al., Nature; Kotani et al., J Biol Chem), and it has since been a molecule widely used as a reference for studying that pathway. In the laboratory it is used as a KISS1R agonist.
Reconstitution and handling
- Work on a clean surface and disinfect the vial stopper with an alcohol wipe before piercing it.
- Add the bacteriostatic water slowly, letting it run down the inner wall of the vial rather than landing on the powder.
- Do not shake. Swirl the vial gently until the lyophilized powder has fully dissolved: vigorous agitation degrades the peptide.
- The solution should come out clear. If it turns cloudy or shows suspended particles, do not use it.
